Publications

Please find Dr. Engel’s Google Scholar Profile here.

Papers

  1. Magda Zaoralová, Joseph Yoniles, Prerna Giri, Richard G. Held, Alison Vander Roest, Peter D. Dahlberg, Daniel Bernstein, Alexander R. Dunn, Leeya Engel; Cryo-Electron Tomography Reveals Nanoscale Thick Filament Disorganization in MYH7 P710R Hypertrophic Cardiomyopathy Cardiomyocytes. ACS Nano 1 September 2026; 20 (34): 23748–23755. https://doi.org/10.1021/acsnano.6c03369
  2. Magdalena Bartolewska, Patrick Martin, Agniva Dutta, Rita Vilensky, Noa Ben-Asher, Leeya Engel, Filippo Pierini, Iyad Khamaysi, Eyal Zussman; Electrostatic Cell Trapping for Enhanced Sample Yield in Brush Cytology. ACS Appl. Mater. Interfaces 18 March 2026; 18 (10): 14660–14673. https://doi.org/10.1021/acsami.5c24675
  3. Nadav Jacobsen, Noa Ben-Asher, Leeya Engel; Characterizing the stability of poly-ethylene glycol surface coatings as a function of environmental conditions for micropatterning applications. J. Vac. Sci. Technol. B 1 March 2026; 44 (2): 024001. https://doi.org/10.1116/6.0004946
  4. Oliver, S.L., Chen, M., Engel, L. et al. Cryogenic electron tomography reveals herpesvirus capsid assembly intermediates inside the cell nucleus. Nat Commun 17, 3197 (2026). https://doi.org/10.1038/s41467-026-69811-4
  5. Yoniles, J., Zaoralová, M., Fajardo, G., Giri, P., Engel, L., Bernstein, D., Dunn, A.R., et al. BPS2026 – Getting to the heart of hypertrophic cardiomyopathy with cryogenic electron tomography. Biophysical Journal 125(4), 9a (2026). https://doi.org/10.1016/j.bpj.2025.11.1010-0
  6. Magda Zaoralová, Joseph Yoniles, Prerna Giri, Richard G. Held, Alison Vander Roest, Peter D. Dahlberg, Daniel Bernstein, Alexander R. Dunn, Leeya Engel.  Cryo-ET reveals nanoscale thick filament disorganization in MYH7 P710R hypertrophic cardiomyopathy cardiomyocytes. bioRxiv 2026.02.18.706593; https://doi.org/10.64898/2026.02.18.706593
  7. Amit Avrahami, Noa Ben Asher, Ran Zalk, Leeya Engel. Gold Electron Microscopy Grids with Anisotropic Foil Geometry Enable On-Grid Contact Guidance.bioRxiv 2026.06.18.733150;  https://doi.org/10.64898/2026.06.18.733150 

  8. Engel, L., Zaoralová, M., Zhou, M. et al. Extracellular filaments revealed by affinity capture cryogenic-electron tomography. Nat Commun 16, 9802 (2025). https://doi.org/10.1038/s41467-025-64795-z

  9. Shani Tcherner Elad, Noa Ben-Asher, Leeya Engel. Cool and collected: Advances in sample preparation for cryo-electron microscopy. Current Opinion in Structural Biology, Volume 94, 2025, 103132, ISSN 0959-440X, https://doi.org/10.1016/j.sbi.2025.103132.
  10. Shani Tcherner Elad, Rita Vilensky, Noa Ben Asher, Nataliya Logvina, Ran Zalk, Eyal Zussman, Leeya Engel. An engineered platform to study the influence of extracellular matrix nanotopography on cell ultrastructure. Nanoscale, 2025, 17, 14687 – 14695 https://doi.org/10.1039/D4NR05508J
  11. Leeya Engel, Kevin J. Liu*, Kiara W. Cui*, Eva L. de la Serna, Vipul T. Vachharajani, Carolyn E. Dundes, Sherry Li Zheng, Manali Begur, Kyle M. Loh, Lay Teng Ang, Alexander R. Dunn (*equal contribution). A microfluidic platform for anterior-posterior human endoderm patterning via countervailing morphogen gradients in vitro. iScience 111744. (2025) https://doi.org/10.1016/j.isci.2025.111744.
  12. R.A. Woldeyes, M. Nishiga, A.S. Vander Roest, L. Engel, et al., Cryo-electron tomography reveals the structural diversity of cardiac proteins in their cellular context. bioRxiv. (2023) https://www.biorxiv.org/content/10.1101/2023.10.26.564098v1
  13. L. Engel, M. Zaoralova, A.R. Dunn, S.L. Oliver. Extracellular filaments revealed by affinity capture cryo-electron tomography of lymphocytes. bioRxiv. (2023) https://doi.org/10.1101/2023.08.05.552110 
  14. Woldeyes RA, Nishiga M, Roest ASV, Engel L, Giri P, Montenegro GC, Dunn AR, Spudich JA, Bernstein D, Schmid MF, Wu JC, Chiu W. Structure of the Thin Filament in Human iPSC-derived Cardiomyocytes and its Response to Heart Disease. doi: 10.1101/2023.10.26.564098.
  16. L. Engel*, C.G. Vasquez*, E.A. Montabana, B.M. Sow, M.P. Walkiewicz, W.I. Weis, A.R. Dunn (*equal contribution). Lattice micropatterning for cryo-electron tomography studies of cell-cell contacts. Journal of Structural Biology 213(4), 107791. (2021) https://doi.org/10.1016/j.jsb.2021.107791
  17. M. Garcia, E. Sadeghipour, L. Engel, W.J. Nelson, B.L. Pruitt. MEMS Device for Applying Shear and Tension to an Epithelium combined with Fluorescent Live Cell Imaging. J Micromech Microeng 30 125004. (2020) https://doi.org/10.1088/1361-6439/abb12c
  18. W. Cui*, L. Engel*, C.E. Dundes, T.C. Nguyen, K.M. Loh, A.R. Dunn (*equal contribution). Spatially controlled stem cell differentiation via morphogen gradients: A comparison of static and dynamic microfluidic platforms. Journal of Vacuum Science & Technology A 38 (033205). (2020) https://doi.org/10.1116/1.5142012
  19. L. Engel, G. Gaietta, L.P. Dow, M.F. Swift, G. Pardon, N. Volkmann, W.I. Weis, D. Hanein, B.L. Pruitt. Extracellular matrix micropatterning technology for whole cell cryogenic electron microscopy studies. J Micromech Microeng 29 (11). (2019) https://doi.org/10.1088/1361-6439/ab419a
  20. L. Engel, C. Liu, N. Hemed, Y. Khan, A. Arias, S. Krylov, Y. Shacham-Diamand, L. Lin. Local electrochemical control of hydrogel microactuators in microfluidics. J Micromech Microeng 28 (10), 105005. (2018) https://doi.org/10.1088/1361-6439/aacc31
  21. M. Ben David, L. Engel, Y. Shacham-Diamand. Spectroscopic Ellipsometry Study of Spin Coated P(VDF-TrFE-CTFE) Thin Films and P(VDF-TrFE-CTFE)/PMMA Blends. Microelectronic Engineering. 17, 37-43. (2017) https://doi.org/10.1016/j.mee.2017.01.030
  22. L. Engel*, K.R. Van Volkinburg*, M. Ben-David, G.N. Washington, S. Krylov, Y. Shacham-Diamand (*equal contribution). Fabrication of a self-sensing electroactive polymer bimorph actuator based on polyvinylidene fluoride and its electrostrictive terpolymer. Proc. SPIE 9798, Electroactive Polymer Actuators and Devices (EAPAD), 2016, Las Vegas, Nevada, USA. https://doi.org/10.1117/12.2218383
  23. R. Feiner, L. Engel, S. Fleisher, M. Malki, A. Shapira, Y. Shacham-Diamand, T. Dvir. Engineered hybrid cardiac patches with multifunctional electronics. Nature Materials. (2016)  https://doi.org/10.1038/nmat4590
  24. K. Hakshur*, L. Engel*, Y. Shacham-Diamand, S. Ruschin (*equal contribution). High surface area thermoplastic polymer films fabricated by mechanical tearing using nano-porous silicon. Microelectronic Engineering 150, 71-73. (2016) https://doi.org/10.1016/j.mee.2015.11.003
  25. L. Engel, S. Kruk, J. Shklovsky, S. Krylov, Y. Shacham-Diamand. A study toward the development of an electromechanical poly(vinylidene fluoride-trifluoroethylene-chlorofluoroethylene) buckling membrane actuator. J Micromech Microeng 24 (12), 125027. (2014) https://doi.org/10.1088/0960-1317/24/12/125027
  26. N. Jackson, P. Verbrugghe, D. Cuypers, K. Adesanya, L. Engel, et al. A Cardiovascular Occlusion Method Based on the use of a Smart Hydrogel. IEEE Transactions on Biomedical Engineering 62 (20), 399-406. (2014) https://doi.org/10.1109/TBME.2014.2353933
  27. L. Engel, S. Krylov, Y. Shacham-Diamand. Thermoplastic nano-imprint lithography of electro active polymer poly(vinylidene fluoride-trifluoroethylene-chlorofluoroethylene) for micro/nano scale sensors and actuators. J Micro/Nanolithography, MEMS, and MOEMS 13(3), 033011-033011. (2014) https://doi.org/10.1117/1.JMM.13.3.033011
  28. L. Engel, O. Berkh, K. Adesanya, J. Shklovsky, P. Dubruel, S. Krylov, Y. Shacham-Diamand. Actuation of a novel Pluronic-based hydrogel: Electromechanical response and the role of applied current. Sensors and Actuators B: Chemical 191, 650-658. (2013) https://doi.org/10.1016/j.snb.2013.10.031
  29. J. Shklovsky*, L. Engel*, Y. Sverdlov, S. Krylov, Y. Shacham-Diamand (*equal contribution). Nano-imprinting lithography of P(VDF-TrFE-CFE) for flexible freestanding MEMS devices. Microelectronic Engineering. 100, 41-46. (2012) https://doi.org/10.1016/j.mee.2012.07.112
  30. L. Engel, J. Shklovsky, D. Schreiber, S. Krylov, Y. Shacham-Diamand. Freestanding smooth micron-scale polydimethylsiloxane (PDMS) membranes by thermal imprinting. J Micromech Microeng 22 (4), 045003. (2012) https://doi.org/10.1088/0960-1317/22/4/045003

Patents granted

  1. F. Stam, N. Jackson, P. Dubruel, K. Adensanya, A. Embrechts, E. Mendes, H.P. Neves, P. Herijgers, P. Verbrugghe, Y. Shacham, L. Engel, S. Krylov. Hydrogel based occlusion systems. U.S. Patent Appl. No.: 13/832,069. (2013)
  2. T. Dvir, Y. Shacham-Diamand, R. Feiner, L. Engel. Electronic Scaffold and Uses Thereof. U.S. Patent Appl. No.: 61/905,230. (2014)

Patents submitted

  1. L. Oliver and L. Engel. Affinity capture system for electron microscopy. U.S. Patent Appl. No.: 63/334470. (2022)